Data availabilityThe raw CUT&Tag sequence data reported in this paper have been deposited in the Genome Sequence Archive (GSA) at the National Genomics Data Center, China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences (GSA: CRA039417). This study made use of the following public data: Drosophila melanogaster genome assembly dm6 (UCSC Genome Browser: https://genome.ucsc.edu/cgi-bin/hgGateway?db=dm6) and Su(H) DamID (GEO: GSM3756569). Source data are provided with this paper.Code availability
The custom code generated in this study is publicly available via the Figshare repository. This includes the pipeline for analysing CUT&Tag sequencing data (https://doi.org/10.6084/m9.figshare.29569391)71, the scripts for quantifying the symmetrical ISC division probability r from clone datasets (https://doi.org/10.6084/m9.figshare.31311136)69, the stochastic simulation framework for modelling EEC production under probabilistic symmetrical divisions (https://doi.org/10.6084/m9.figshare.31311127)70 and the R scripts for data visualization of the relative position and clone distribution of new EECs (https://doi.org/10.6084/m9.figshare.32617422)72.
ReferencesCarrelha, J. et al. Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells. Nature 554, 106–111 (2018).Article
