Author informationAuthor notesJasper S. PhelpsPresent address: Neuroengineering Laboratory, Brain Mind Institute and Institute of Bioengineering, EPFL, Lausanne, SwitzerlandMinsu KimPresent address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USAMinsu KimPresent address: Center for Brain Science, Harvard University, Cambridge, MA, USAFarzaan SalmanPresent address: Department of Neurobiology, Harvard Medical School, Boston, MA, USARyan T. MaloneyPresent address: Psychology Department, Colorado College, Colorado Springs, CO, USADimitrios Stasi GiakoumasPresent address: Interdisciplinary Graduate Program in Neuroscience, University of Iowa, Iowa City, IA, USAThese authors contributed equally: Alexander S. Bates, Jasper S. Phelps, Minsu Kim, Helen H. YangAuthors and AffiliationsDepartment of Neurobiology, Harvard Medical School, Boston, MA, USAAlexander S. Bates, Jasper S. Phelps, Minsu Kim, Helen H. Yang, Zaki Ajabi, Kevin M. Delgado, Mohammed Abdal Monium Osman, Sophia Renauld, Janki Patel, Matthew F. Collie, Jingxuan Fan, Diego A. Pacheco, Yunzhi Zhao, Wenyi Zhang, Jasper S. Phelps, Minsu Kim, Emily Ye, Jan Drugowitsch, Rachel I. Wilson & Wei-Chung Allen LeeCentre for Neural Circuit and Behaviour, University of Oxford, Oxford, UKAlexander S. BatesPrinceton Neuroscience Institute, Princeton University, Princeton, NJ, USAArie Matsliah, Christopher K. Salmon, Jay Gager, Benjamin Silverman, Szi-chieh Yu, Amy R. Sterling, Marissa Sorek, Serene Dhawan, Austin Burke, James Hebditch, Kyle P. Willie, Ryan Willie, Emily Behnke, Sandeep Kumar, Anna Verbe, Zijin Huang, H. Sebastian Seung & Mala MurthyYikes LLC, Baltimore, MD, USAEric PerlmanDepartment of Biology, West Virginia University, Morgantown, WV, USAFarzaan Salman, Farzaan Salman & Kevin C. DalyAelysia LTD, Bristol, UKLaia Serratosa Capdevila, Ruairí J. V. Roberts, Eva J. Munnelly, Nina Griggs, Helen Langley & Borja Moya-LlamasBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, ChinaZuoyu Zhang & Tatsuo S. OkuboChinese Institute for Brain Research, Beijing, Beijing, ChinaZuoyu Zhang & Tatsuo S. OkuboDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USARyan T. Maloney, Ryan T. Maloney, Benjamin L. de Bivort & Benjamin L. de BivortCenter for Brain Science, Harvard University, Cambridge, MA, USARyan T. Maloney, Ryan T. Maloney, Benjamin L. de Bivort & Benjamin L. de BivortEyewire, Boston, MA, USAKrzysztof Kruk, Nikitas Serafetinidis, Doug Bland, Anne Kristiansen, Jaime Skelton, Tom Stocks, Anthony Hernandez, Devon Jones & Celia DavidGenetics Department, Leipzig University, Leipzig, GermanyFinja Klemm & Katharina EichlerZoology Department, University of Cambridge, Cambridge, UKPaul BrooksDepartment of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, USAEllen LesserDepartment of Neurobiology and Biophysics, University of Washington, Seattle, WA, USAJessica M. Jones, Sara E. Pierce-Lundgren, Su-Yee Lee, Yichen Luo, John C. Tuthill & Anthony AzevedoSchool of Neuroscience, Virginia Tech, Blacksburg, VA, USAAndrew P. Cook & Sweta AgrawalDepartment of Biology, University of Nevada Reno, Reno, NV, USATheresa H. McKimDepartment of Biology, University of Florida, Gainesville, FL, USADimitrios Stasi Giakoumas, Dimitrios Stasi Giakoumas & Zepeng YaoMolecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USABenjamin Gorko & Dustin GarnerHHMI Janelia, Ashburn, VA, USAJustin Ellis-Joyce, Diane-Yayra Adjavon & Jan FunkeDepartment of Neuroscience, Johns Hopkins University, Baltimore, MD, USAJustin Ellis-JoyceInstitute of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, ChinaJiayi Zhang & Fei WangDepartment of Biological Sciences, Vanderbilt University, Nashville, TN, USAEmily C. Kophs & Marie P. SuverMax Planck Institute for Biological Intelligence, Martinsried, GermanyTjalda Falt & Lisa M. FenkInstitute of Neurobiology, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto RicoAlexa M. Negron-Morales, Gabriel A. Nieves-Sanabria, Omaris Y. De Pablo-Crespo, Fernando J. Figueroa Santiago, Andrew M. Seeds & Stefanie HampelZetta AI LLC, Sherrill, NY, USASergiy Popovych, Nico Kemnitz, Dodam Ih, Kisuk Lee, Ran Lu, Akhilesh Halageri, J. Alexander Bae & Thomas MacrinaSchool of Informatics, University of Edinburgh, Edinburgh, UKBen JourdanJapan Advanced Institute of Science and Technology (JAIST), Nomi, JapanGregory SchwartzmanMolecular Brain Physiology and Behavior, LIMES Institute, University of Bonn, Bonn, GermanyDamian D. Demarest & Michael J. PankratzDepartment of Neuroscience, West Virginia University, Morgantown, WV, USAKevin C. DalyAllen Institute for Brain Science, Seattle, WA, USASven Dorkenwald & Forrest CollmanFlorida Chemical Senses Institute, University of Florida, Gainesville, FL, USAZepeng YaoMcKnight Brain Institute, University of Florida, Gainesville, FL, USAZepeng YaoGenetics Institute, University of Florida, Gainesville, FL, USAZepeng YaoDepartment of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USAStephen J. HustonNeurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UKTomke Stürner & Gregory S. X. E. JefferisDepartment of Biochemistry and Molecular Biology, University of Nevada Reno, Reno, NV, USAMeet ZandawalaNeurobiology and Genetics, Theodor-Boveri-Institute, Biocenter, Julius-Maximilians-University of Würzburg, Am Hubland, Würzburg, GermanyMeet ZandawalaComputer Science Department, Princeton University, Princeton, NJ, USAH. Sebastian SeungF.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USAWei-Chung Allen LeeChampalimaud Neuroscience Programme, Champalimaud Centre for the Unknown, Lisbon, PortugalSofia PintoDepartment of Biology, Leipzig University, Leipzig, GermanyWolf HuetterothRWTH Aachen University, Aachen, GermanyZequan LiuAuthorsAlexander S. BatesJasper S. PhelpsMinsu KimHelen H. YangArie MatsliahZaki AjabiEric PerlmanKevin M. DelgadoMohammed Abdal Monium OsmanChristopher K. SalmonJay GagerBenjamin SilvermanSophia RenauldFarzaan SalmanJanki PatelMatthew F. CollieJingxuan FanDiego A. PachecoYunzhi ZhaoWenyi ZhangLaia Serratosa CapdevilaRuairí J. V. RobertsEva J. MunnellyNina GriggsHelen LangleyBorja Moya-LlamasZuoyu ZhangRyan T. MaloneySzi-chieh YuAmy R. SterlingMarissa SorekKrzysztof KrukNikitas SerafetinidisSerene DhawanFinja KlemmPaul BrooksEllen LesserJessica M. JonesSara E. Pierce-LundgrenSu-Yee LeeYichen LuoAndrew P. CookTheresa H. McKimDimitrios Stasi GiakoumasBenjamin GorkoJustin Ellis-JoyceJiayi ZhangEmily C. KophsTjalda FaltAlexa M. Negron-MoralesAustin BurkeJames HebditchKyle P. WillieRyan WillieSergiy PopovychNico KemnitzDodam IhKisuk LeeRan LuAkhilesh HalageriJ. Alexander BaeBen JourdanGregory SchwartzmanDamian D. DemarestEmily BehnkeDoug BlandAnne KristiansenJaime SkeltonTom StocksDustin GarnerAnthony HernandezSandeep KumarKevin C. DalySven DorkenwaldForrest CollmanMarie P. SuverLisa M. FenkMichael J. PankratzZepeng YaoFei WangStephen J. HustonTomke StürnerGregory S. X. E. JefferisKatharina EichlerAndrew M. SeedsStefanie HampelSweta AgrawalTatsuo S. OkuboMeet ZandawalaThomas MacrinaDiane-Yayra AdjavonJan FunkeJohn C. TuthillAnthony AzevedoH. Sebastian SeungBenjamin L. de BivortMala MurthyJan DrugowitschRachel I. WilsonWei-Chung Allen LeeConsortiaThe BANC-FlyWire ConsortiumAlexander S. Bates, Jasper S. Phelps, Minsu Kim, Helen H. Yang, Arie Matsliah, Zaki Ajabi, Eric Perlman, Kevin M. Delgado, Mohammed Abdal Monium Osman, Christopher K. Salmon, Jay Gager, Benjamin Silverman, Sophia Renauld, Farzaan Salman, Janki Patel, Matthew F. Collie, Jingxuan Fan, Diego A. Pacheco, Yunzhi Zhao, Wenyi Zhang, Laia Serratosa Capdevila, Ruairí J. V. Roberts, Eva J. Munnelly, Nina Griggs, Helen Langley, Borja Moya-Llamas, Zuoyu Zhang, Ryan T. Maloney, Szi-chieh Yu, Amy R. Sterling, Marissa Sorek, Krzysztof Kruk, Nikitas Serafetinidis, Serene Dhawan, Finja Klemm, Paul Brooks, Ellen Lesser, Jessica M. Jones, Sara E. Pierce-Lundgren, Su-Yee Lee, Yichen Luo, Andrew P. Cook, Theresa H. McKim, Dimitrios Stasi Giakoumas, Benjamin Gorko, Justin Ellis-Joyce, Jiayi Zhang, Emily C. Kophs, Tjalda Falt, Alexa M. Negron-Morales, Austin Burke, James Hebditch, Kyle P. Willie, Ryan Willie, Sergiy Popovych, Nico Kemnitz, Dodam Ih, Kisuk Lee, Ran Lu, Akhilesh Halageri, J. Alexander Bae, Ben Jourdan, Gregory Schwartzman, Damian D. Demarest, Emily Behnke, Doug Bland, Anne Kristiansen, Jaime Skelton, Tom Stocks, Dustin Garner, Anthony Hernandez, Sandeep Kumar, Kevin C. Daly, Sven Dorkenwald, Forrest Collman, Marie P. Suver, Lisa M. Fenk, Michael J. Pankratz, Zepeng Yao, Fei Wang, Stephen J. Huston, Tomke Stürner, Gregory S. X. E. Jefferis, Katharina Eichler, Andrew M. Seeds, Stefanie Hampel, Sweta Agrawal, Tatsuo S. Okubo, Meet Zandawala, Thomas Macrina, Diane-Yayra Adjavon, Jan Funke, John C. Tuthill, Anthony Azevedo, H. Sebastian Seung, Benjamin L. de Bivort, Mala Murthy, Jan Drugowitsch, Rachel I. Wilson, Wei-Chung Allen Lee, Anna Verbe, Gabriel A. Nieves-Sanabria, Devon Jones, Zijin Huang, Sofia Pinto, Celia David, Omaris Y. De Pablo-Crespo, Emily Ye, Wolf Huetteroth, Zequan Liu & Fernando J. Figueroa SantiagoCorresponding authorsCorrespondence to