Structural basis for chaperone-guided assembly of RNA-induced silencing complex - Nature

Data availabilityThe cryo-EM structure of AMC–let-7a-1 has been deposited in the PDB under accession code 9W5I. The composite map and consensus map of AMC–let-7a-1 have been deposited in the EMDB under accession codes EMD-65663 and EMD-65662, respectively. The partial maps of local refinement focused on MID/PIWI, N-domain and HSP90–p23 have been deposited in the EMDB under accession codes EMD-65661, EMD-65660 and EMD-65659, respectively. The consensus map and AGO2-focused map of AMC have been deposited in the EMDB under accession codes EMD-69544 and EMD-65658, respectively. There are no restrictions on data availability. For material requests, please contact the corresponding authors. Other structural models cited in this study for analysis (4W5N, 4W5O, 7KRJ, 7XW2, 8EOB, 9CMP, 7V6C, 7ZUB, 8FFW, 5FWK, 5FWL, 7Z37 and 8GFT) are also available at the PDB.ReferencesIwasaki, S. et al. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Mol. Cell 39, 292–299 (2010).Article

PubMed

Google Scholar

Miyoshi, T., Takeuchi, A., Siomi, H. & Siomi, M. C. A direct role for Hsp90 in pre-RISC formation in Drosophila. Nat. Struct. Mol. Biol. 17, 1024–1026 (2010).Article