Buffalo and San Francisco are the sixth seed in their respective conference
The San Francisco 49ers came from behind twice in the final quarter to knock out defending champions Philadelphia as a thrilling NFL play-off series continued on Sunday.
The Eagles were the NFC's third seed so had home-field advantage for Wildcard Weekend but fell at the first hurdle as the sixth seed 49ers produced a 23-19 upset.
The AFC's sixth seed also won on the road, with the Buffalo Bills claiming a late 27-24 win at third seed Jacksonville.
That kept alive Josh Allen's hopes of reaching his first Super Bowl, with the NFL's reigning Most Valuable Player having put the Bills back in front with one minute four seconds remaining.
