Rational design of disordered proteins for sequence–function investigation - Nature

Data availability

Data and analysis scripts used for figures and analysis in this paper are available at GitHub (https://github.com/sukeniklab/GOOSE_2026 and https://github.com/holehouse-lab/supportingdata/tree/master/2026/GOOSE_2026). Raw sequencing data have been deposited at Zenodo82 (https://zenodo.org/records/18774462).

Code availability

GOOSE is fully open source and is available at GitHub (https://github.com/idptools/goose/). Documentation for using GOOSE is available online (https://goose.readthedocs.io/en/latest/). A subset of GOOSE’s functionality is available across two Colab Notebooks. For sequence generation and variant design, use the following notebook: https://colab.research.google.com/drive/1U9B-TfoNEZbbjhPUG5lrMPS0JL0nDB3o?usp=sharing. For creating sequences with specific homotypic and/or heterotypic IDR–IDR interactions, use the following notebook: https://colab.research.google.com/drive/1aJajo1IK66ApFSMwCCBumCZixlfshw7A?usp=sharing.

ReferencesHolehouse, A. S. & Kragelund, B. B. The molecular basis for cellular function of intrinsically disordered protein regions. Nat. Rev. Mol. Cell Biol. 25, 187–211 (2024).Article