England defender Djed Spence appeared to be the only player in the squad who did not shake Thomas Partey's hand before England's World Cup clash with Ghana in Boston.
Attention had focused on the pre-match handshake amid the legal case involving the Ghana midfielder.
Partey is due to stand trial at Southwark Crown Court next year after being charged with multiple counts of rape and one count of sexual assault. He has denied all allegations. His lawyer has said the former Arsenal player welcomes the opportunity to clear his name.
Television cameras cut away during the handshake line, but footage circulating online appeared to show Spence keeping his right hand in the pocket of his tracksuit top as Partey approached.
The moment appeared to surprise Partey, who briefly looked back towards the Tottenham defender before continuing down the line.
