James Middleton joined glamorous Lady Amelia and Eliza Spencer for London Air Ambulance's star-studded charity gala last night. The Spencer sisters , who are the late Princess Diana's nieces, looked elegant at the glittering black and white gala dinner at Raffles London at the OWO (Old War Office).The socialite twins, both 33, exuded sophistication and glamour as they stunned in floor-length gowns for the prestigious event, which sought to celebrate the incredible work of the London-based charity. While Lady Eliza opted for a striking black floor lace gown and accessorised with a diamond necklace and simple handbag. Meanwhile, Lady Amelia's show-stopping embezzled dress was dominated by glistening diamonds, accompanied by a matching purse and necklace. The two sisters both wore their long tresses down, and beamed as they posed for photographers at London's elegant OWO hotel.Also in attendance at the annual charity gala were Liz Hurley and Billy Ray Cyrus, alongside Sadie Frost.The Prince of Wales, who is patron of the charity, also provided a foreword for the programme in which he reflected on is days as an air ambulance pilot and expressed how 'honoured' he felt to represent the incredible life-saving service. Lady Eliza Spencer and Lady Amelia Spencer , who are the late Princess Diana's nieces, looked elegant at the glittering black and white gala dinner at Raffles London at the OWO (Old War Office) James Middleton, brother of the Princess of Wales was also in attendance at the event with his wife, Alizee Thevenet (both pictured). The Prince of Wales, who is patron of the charity, provided a foreword in which he reflected on is days as an air ambulance pilot Pictured: Elizabeth Hurley and Billy Ray Cyrus attend the charity gala event which was held at Raffles London at the OWO (Old War Office)'During my time as an air ambulance pilot, I took great pride in being part of a team that delivered such vital work, knowing that in a small way I could play a role in giving someone a chance of survival,' he said.'Now, as Patron of London's Air Ambulance Charity, I am honoured to represent the service and help raise awareness of its life saving work. 'Whatever you do for the charity whether on the frontline, a charity employee, a volunteer or a supporter you are part of the team that makes sure when someone is critically injured, expert medical care can arrive within minutes.'The Prince of Wales - who served as an air ambulance pilot in East Anglia - has been patron of the organisation since 2020.Jonathan Jenkins, Chief Executive of London's Air Ambulance Charity, said: 'We are enormously grateful to His Royal Highness The Prince of Wales for his continued patronage and heartfelt words of support, and to every guest, donor and supporter who joined us at the Black & White Gala.'Demand for our service continues to grow, and the generosity shown this evening will make a real and lasting difference to the patients and families who rely on us every day, helping loved ones share more moments together.'Julia Leal Hartogs, Chair of the London's Air Ambulance Charity Philanthropy Board, said: 'Tonight was a powerful reminder of the impact we can have when we come together.'The funds raised will help London's Air Ambulance Charity continue delivering life-saving trauma care across the capital, giving critically injured patients the best possible chance of survival.'A huge thank you to everyone who helped make it possible, from our attendees to our performers and everyone who donated a prize for our auction – including our fabulous auctioneer Lord Harry Dalmeny.' Sadie Frost was also in attendance at the event - which sought to celebrate the incredible work of the charity. The organisation has so far raised well over £1million on the night to help fund its life-saving service The annual event featured a star-studded guest list. The Prince of Wales - who served as an air ambulance pilot in East Anglia - has been patron of the organisation since 2020It comes after the Spencer sisters put on a brave face as they attended Cannes Film Festival after 'missing' their 'estranged' father's wedding.The socialites oozed glamour in shimmering gowns as they joined a host of A-listers at the screening of Coward, the queer First World War drama, on Thursday.Their high-profile outing comes after their father Earl Spencer, 62, wed his Norwegian archaeologist partner Professor Cat Jarman, 44, in a secret ceremony in Arizona earlier this month.However, it's thought that Eliza and Amelia didn't attend the low-key nuptials amid speculation of a fallout between the Spencer sisters and the father-of-seven.The Daily Mail's Richard Eden suggested some of the Earl's own children were told about the marriage after he had already exchanged vows with Cat. ‘They found out via a text,’ claims a family friend.Rumours of a family rift began in July 2021, when Charles skipped his eldest daughter Lady Kitty's Italian wedding to retail millionaire Michael Lewis and failed to post about the family occasion on his social media accounts.Instead, Lady Kitty - who is 32 years younger than her husband - was walked down the aisle by her brothers Louis Spencer, Viscount Althorp, and Samuel Aitken, whose father is businessman Jonathan Aitken.Earl Spencer was also absent from Lady Amelia's mountaintop wedding to lifestyle coach Greg Mallet in Stellenbosch, South Africa, in 2023.