The wife of a Kentucky sheriff accused of shooting dead a judge in his own chambers has defended her daughter to deny she had any relationship with the married victim.

Letcher County Sheriff Shawn 'Mickey' Stines has been behind bars awaiting trial since the September 19 death of his longtime friend, District Judge Kevin Mullins.

Extraordinary video appeared to capture the moment a calm Stines shot Mullins dead inside his own chambers, but he has maintained his innocence and pleaded not guilty.

After the shocking incident, rumors swirled about Mullins' connection to Stines' minor daughter when her number appeared on his phone.

But the young girl and Stines' wife Caroline vehemently denied the suggestions.